Blog • HighRes

How Scientists Built 3 Production-Ready HTS Automation Systems in One Year

Written by HighRes Biosolutions | Mar 30, 2026 11:00:00 AM

HighRes partners with life science organizations to implement intelligent data and lab automation workflows that empower every team member to plan and execute efficient and reproducible science. At SLAS2026, HighRes was joined by three distinguished partner organizations — Enveda, Pfizer, and Merck — each sharing their real-world experiences building and evolving high-throughput screening and sample management platforms powered by HighRes automation.

This customer spotlight series features the stories and insights shared by each partner, offering a window into the practical challenges, strategic decisions, and operational outcomes that define world-class lab automation in drug discovery.

Customer Spotlight: Pfizer

Presented by Rich Frisbie, Principal Scientist, High-Throughput Screening, Discovery Biology & Pharmacology

About the Pfizer HTS Group

Pfizer's High-Throughput Screening lab resides within the Discovery Biology and Pharmacology group in Groton, Connecticut, supporting all of Pfizer's early drug discovery portfolio. The group spans HTS, SAR labs, cell and protein reagent teams, and works across multiple therapeutic disease areas. Rich Frisbie has been part of small molecule drug discovery at Pfizer for almost 20 years.

The lab is a newer build, with the team growing rapidly from a small founding group into a fully equipped, production-ready HTS facility. Rich's presentation focused specifically on lessons learned while designing, constructing, and commissioning three major HighRes automation systems over the past year-plus.

The Build Journey

Pfizer's automation journey began with a detailed retrospective evaluation of existing infrastructure and a clear set of forward-looking goals. Key requirements included:

  • Adoption of 1536-well format to leverage existing HighRes equipment
  • Integration of legacy carts — some up to 12 years old — with brand new instrumentation
  • Built-in flexibility and redundancy, including the ability to traverse carts across multiple lab systems
  • Full EH&S compliance, including HEPA-enclosed cell-based work environments and chemical exhaust venting for paraformaldehyde staining

The design phase with HighRes spanned into early 2024, with biweekly meetings to develop concepts. A concurrent lab renovation involving raised ceilings, new walls, plumbing, electrical, and data infrastructure added complexity but was managed in parallel with the system design process.

Three Systems Built

Pfizer commissioned three major Nucleus® automation systems, each designed with intentional flexibility and upgradability:

1. The ZCell System

The largest and most complex of the three systems, the ZCell houses approximately 25 instruments, including dual centrifuges, dual PlateLocs, multiple Q1 BioShakes, Revvity readers, and a Prime®Liquid Handler currently configured for advanced plate storage. The system features three dockable cart configurations and integrates a SteriStore for cell-based work. All cell-based operations, including de-lidding, are performed within a HEPA-enclosed environment for EH&S compliance.

2. The Tri-Doc System

Born from the commissioning of the Echo FlexCart, a large, ~600-pound mobile unit, the Tri-Doc takes a unique approach: there is no central robotic arm. The ACell arm travels with the FlexCart itself, docking to the system and interfacing with adjacent carts. In its current configuration it supports biochemical HTS, with Prime Liquid Handler providing gantry-style plate storage for up to 100-plate assays. The system is also used extensively for plate reformatting and source plate preparation.

3. The ACell Rail Imaging System

Designed primarily for fully automated online fixing and staining of cells, the ACell Rail includes a chemically vented enclosure for paraformaldehyde work, a HEPA-enclosed Tempest dispenser for cell-based workflows, and Lyconic temperature-controlled storage (capable of handling heavy metal lids that cause issues with standard AmbiStore/SteriStore units). The system supports up to 88 plates of daily imaging throughput, with the flexibility to run concurrent workflows such as qPCR alongside imaging.

 

Video 1. Pfizer’s Automation Journey

Lessons Learned


Rich shared several hard-won lessons for teams embarking on similar automation journeys:

  • Time is the most underestimated factor. Renovation, procurement, EH&S review, and IT integration all run in parallel and take longer than expected.
  • FAT and SAT protocol design is critical. Working closely with HighRes, particularly HighRes automation engineers, to design rigorous factory and site acceptance tests enabled the team to go directly into production screening within days of commissioning.
  • Legacy equipment can be integrated. With minor modifications, carts up to 10 years old were successfully commissioned onto new HighRes systems.
  • Remote access infrastructure is essential. With systems distributed across a large lab, RDC/Net Support access to all system PCs is required for diagnostics, driver updates, and troubleshooting.
  • Electrical planning matters. New carts often require 30-amp twist-lock connections, requiring lab renovations to include appropriate power drops in all cart parking locations.
  • Data integration must be planned upfront. Pfizer's data flows from readers through Cellario® to cloud storage and shared drives — this required dedicated data ports and network planning during the renovation phase.

Key Outcomes

  • Three production-ready HTS systems commissioned within a newly renovated lab
  • Cell-based and biochemical assay capabilities running in parallel across redundant systems
  • Fully automated fixing and staining workflow live for online high-content imaging
  • Near-immediate production screening following SAT completion

Download a copy of Rich’s slides here.

Questions?

Contact our applications and technology experts for personalized advice, recommendations, and demonstrations! Learn more about Pfizer or reach out to Rich about his systems.